Modified yeast to boost biofuel production

Washington, March 29 (ANI): A team of scientists has worked out how to modify yeast cells so that they successfully convert a wider range of sugars from plant waste such as wheat and rice straw into alcohol that can be used as biofuel.

The scientists in question are Dr Christian Weber and Professor Eckhard Boles from Frankfurt University, Germany.

Bioalcohols produced by microbial fermentations are an example of second-generation biofuels that use raw materials not used in food production.

Plant waste is available in large amounts and contains a mixture of complex sugars including hexoses and pentoses that can be fermented to alcohol.

“As these feedstocks represent the biggest portion of processing costs, we need rapid and efficient conversion of all sugars present. At the moment there is a lack of microbes that will efficiently convert both hexoses and pentoses into ethanol,” explained Dr Weber.

Bakers’ yeast Saccharomyces cerevisiae is already used in the beverage industry to efficiently convert hexose sugars, such as glucose, into ethanol.

By transferring genes from bacteria that naturally break down pentose, Dr Weber’s team have engineered S. cerevisiae to successfully ferment pentose and hexose sugars.

“As pentoses represent a substantial part of the feedstock, the engineered yeast gives a much higher yield of ethanol for the same amount of feedstock,” he said.

To enhance their biofuel potential even more, the yeast is being further modified to produce another bioalcohol – butanol instead of ethanol.

“Compared to ethanol, butanol shows superior properties as a potential biofuel. It has a lower vapour pressure, ignites at a higher temperature and is less corrosive. Butanol could replace fossil fuels up to 100 percent without modifying existing engines,” said Professor Boles. (ANI)

New test to detect tainted milk

Washington, Sept 13 (ANI): Researchers have developed a simple test that would help detect tainted milk within few hours.

Amer AbuGhazaleh, from Southern Illinois University Carbondale’s College of Agricultural Sciences, and Salam Ibrahim, a food microbiologist from North Carolina Agricultural and Technical State University, have shown that the combination of certain bacteria and a common purple dye can reveal the presence of toxins in milk in just a few hours.

“To date, detecting the presence of toxins or pesticides has only been possible by sending samples to a laboratory and waiting a few days for the results,” said AbuGhazaleh.

“An important step toward improving the safety of our dairy supply would be the development of an effective, simple and rapid test that would allow farmers or processors to detect the presence of foreign substances,” the expert added.

During the study, the scientists decided to focus on the bacteria that ferment lactose (milk’s sugars), producing lactic acid.

“For one thing, these bacteria already exist in milk, so if you add some, you’re not doing anything strange,” said AbuGhazaleh.

“Second, they produce a change over time (the lactic acid) that we could monitor. If we didn’t see the change, we would know something was wrong,” the expert said.

They began in 2008 with a few bacterial strains they already had and cyanide, also readily available. Experiments showed not only that the toxin could slow or stop lactic acid production but that this effect increased with the toxic load. Further, the effect appeared in less than four hours.

They then added purple dye to milk samples containing both toxins and bacteria and to samples containing only bacteria.

After eight hours, dye in the non-toxic milk turned yellow, indicating the presence of increased lactic acid, while dye in the toxin-laden milk retained its original purple.

“This kind of colour test could be performed by farmers themselves,” AbuGhazleh said.

“They could add the bacteria and the dye to a sample, leave it alone for a little while and then come back to see if there is any change in the color. If there isn’t, there are problems with the milk,” he added. (ANI)

Soon, a breath mint made from coffee

Washington, June 25 (ANI): A coffee extract can inhibit the growth of bacteria that leads to bad breath, according to a new research from Tel Aviv University.

New laboratory tests have shown that the extract prevents malodorous bacteria from making their presence felt – or smelt.

“Everybody thinks that coffee causes bad breath and it’s often true, because coffee, which has a dehydrating effect in the mouth, becomes potent when mixed with milk, and can ferment into smelly substances,” says breath specialist Prof. Mel Rosenberg of TAU’s Sackler Faculty of Medicine.

But that is not always true. “Contrary to our expectations, we found some components in coffee that actually inhibit bad breath,” Rosenberg added.

In the laboratory, the researchers monitored the bacterial odour production of coffee in saliva.

In the study, three different brands of coffee were tested: the Israeli brand Elite coffee, Landwer Turkish coffee, and Taster’s Choice.

Rosenberg expected to demonstrate the malodour-causing effect of coffee in an in vitro saliva assay developed by Dr. Sarit Levitan in his laboratory. To his surprise, the extracts had the opposite effect.

“The lesson we learned here is one of humility. We expected coffee would cause bad breath, but there is something inside this magic brew that has the opposite effect,” Rosenberg.

Rosenberg would love to isolate the bacterial-inhibiting molecule in order to reap the biggest anti-bacterial benefits from coffee.

According to researchers, the new study could be the foundation for an entirely new class of mouthwash, breath mints and gum.

Purified coffee extract can be added to a breath mint to stop bacteria from forming, stopping bad breath at its source, instead of masking the smell with a mint flavour, the authors said.

The findings were presented to members of the International Society for Breath Odour Research in Germany. (ANI)