Vaccine for urinary tract infections comes closer to reality

Washington, Sept 18 (ANI): A simple vaccine may soon be available to protect against urinary tract infections, thanks to researchers from University of Michigan.

The study conducted over mice showed that the vaccine prevented infection and produced key types of immunity.

It alerts the immune system to iron receptors on the surface of Escherichia coli bacteria that perform a critical function allowing infection to spread.

Administered in the nose, it induces an immune response in the body’s mucosa, a first line of defense against invading pathogens. The response, also produced in mucosal tissue in the urinary tract, should help the body fight infection where it starts.

The researchers used novel systematic approach, combining bioinformatics, genomics and proteomics, to look for key parts of the bacterium that could be used in a vaccine to elicit an effective immune response.

The team, led by Dr. Harry L.T. Mobley, screened 5,379 possible bacterial proteins and identified three strong candidates to use in a vaccine to prime the body to fight E. coli.

Mobley’s team is currently testing more strains of E. coli obtained from women treated at U-M.

If the robust immunity achieved in mice can be reproduced in humans, it could be the first ever vaccine for urinary tract infections.

Most of the strains produce the same iron-related proteins that can be vaccine targets, an encouraging sign that the vaccine could work against many urinary tract infections.

The findings are published in the open-access journal PLoS Pathogens. (ANI)

Spanking found to have negative effects on low-income toddlers

Washington, September 16 (ANI): Spanking negatively affects the behaviour of toddlers in low-income families, according to a new study.

Published in the journal Child Development, the longitudinal study looked at how low-income parents discipline their young children.

It showed that spanking 1-year-olds leads to more aggressive behaviours and less sophisticated cognitive development in the next two years.

Verbal punishment, however, was not found to be associated with such effects, especially when it was accompanied by emotional support from mothers.

Besides, 1-year-olds’ fussiness predicted spanking and verbal punishment at ages 1, 2, and 3.

The study explored whether mothers’ behaviours lead to problematic behaviour in children, whether children’s challenging behaviours elicit harsher discipline, or both.

It looked at more than 2,500 exclusively low-income White, African American, and Mexican-American mothers and their young children, interviewing and observing them at home when the children were 1, 2, and 3 years old.

All participants’ family incomes were at or below the federal poverty level.

Using their own interpretations of spanking, mothers reported how often anyone in the home had spanked their children in the past week.

The study also looked at how often mothers verbally punished-scolded, yelled, or made negative comments-their children.

It showed that African American children were spanked and verbally punished significantly more than the other children in the study.

The authors speculated that that might be due to cultural factors, such as belief in the importance of children’s respect for elders and in the value of physical discipline to instil that respect.

Moreover, some African American mothers said that in preparing their children for a harsh, physically dangerous, and racially discriminating world, there was little room for error in their childrearing.

The study also shed light on information about the effects of such types of discipline.

“Our findings clearly indicate that spanking affects children’s development,” said Lisa J. Berlin, research scientist at the Center for Child and Family Policy at Duke University and the study’s lead author.

Specifically, children who were spanked more often at 1 behaved more aggressively when they were 2, and had lower scores on tests measuring thinking skills when they were 3.

Similar findings were made even after taking into consideration such family characteristics as mothers’ race and ethnicity, age, and education; family income and structure; and the children’s gender.

The study also found that children who were more aggressive at age 2, and had lower cognitive development scores at ages 1 and 2, were not spanked more at ages 2 and 3.

“So the mothers’ behaviours look more influential than the children’s,” said Berlin.

Unlike spanking, however, verbal punishment alone didn’t affect either children’s aggression or their cognitive development.

Interestingly, when verbal punishment was accompanied by emotional support from moms, the children did better on the tests of cognitive ability. (ANI)

Kings of Leon ‘swear at audience’

London, Aug 31 (ANI): American rock band Kings of Leon reportedly swore at fans during their headline performance at the Reading Festival in the UK.

The band not only failed to click with the audience but also struggled with problems in the sound equipment and windy conditions.

Lead singer Caleb Followill started the rant when he hissed out at the bored fans, reports the Daily Star.

He asked fans: “I thought this was supposed to be the loudest crowd in the world?” before pleading with fans to “help us do this” as they launched into their hit Sex On Fire.

However, unable to elicit any reaction from the audience Caled lost his cool.

He raged: “We know you’re sick of Kings of Leon. We’re f***ing sick of Kings of Leon too. But we get up here every night and I thank God for everything I’ve had.

“So for all those who don’t give a f**k about us, I understand. But we’ve worked hard to be here. We’re the goddamn Kings of Leon, so f**k you.”

Caleb and brother Jared then smashed their guitars into the floor, threw the remains into the crowd and walked off the stage gesturing angrily. (ANI)

Lasers can be used to lengthen quantum bit memory by 1,000 times

Washington, June 25 (ANI): Physicists have found that lasers can be used to drastically prolong the shelf life of quantum bit memory, the 0s and 1s of quantum computers, by 1,000 times.

These precarious bits, formed in this case by arrays of semiconductor quantum dots containing a single extra electron, are easily perturbed by magnetic field fluctuations from the nuclei of the atoms creating the quantum dot.

This perturbation causes the bits to essentially forget the piece of information they were tasked with storing.

A quantum dot is a semiconductor nanostructure that is one candidate for creating quantum bits.

The scientists, including the University of Michigan’s Duncan Steel, used lasers to elicit a previously undiscovered natural feedback reaction that stabilizes the quantum dot’s magnetic field, lengthening the stable existence of the quantum bit by several orders of magnitude, or more than 1,000 times.

Because of their ability to represent multiple states simultaneously, quantum computers could theoretically factor numbers dramatically faster and with smaller computers than conventional computers.

For this reason, they could vastly improve computer security.

“In our approach, the quantum bit for information storage is an electron spin confined to a single dot in a semiconductor like indium arsenide,” said Steel.

“One of the serious problems in quantum computing is that anything that disturbs the phase of one of these spins relative to the other causes a loss of coherence and destroys the information that was stored,” he added.

A major cause of information loss in a popular class of semiconductors called 3/5 materials is the interaction of the electron (the quantum bit) with the nuclei of the atoms in the quantum dot holding the electron.

Trapping the electron in a particular spin, as is necessary in quantum computers, gives rise to a small magnetic field that couples with the magnetic field in the nuclei and breaks down the memory in a few billionths of a second.

By exciting the quantum dot with a laser, the scientists were able to block the interaction of these magnetic fields.

The laser causes an electron in the quantum dot to jump to a higher energy level, leaving behind a charged hole in the electron cloud.

This hole, or space vacated by an electron, also has a magnetic field due to the collective spin of the remaining electron cloud.

It turns out that the hole acts directly with the nuclei and controls its magnetic field without any intervention from outside except the fixed excitation by the lasers to create the hole. (ANI)

Understanding plants’ immune system will help researchers build better crops

Washington, May 28 (ANI): Researchers at the University of Missouri, US, have identified important suppressors that negatively regulate the responses of the immune system in the plant species Arabidopsis thaliana, which would allow breeders to create better yielding crop plants.

“The immune system provides plants with strong protection from pathogens,” said Walter Gassmann, associate professor of plant sciences in the MU Christopher S. Bond Life Sciences Center and the College of Agriculture, Food and Natural Resources.

“However, this response has the potential to be highly deleterious to the plant and needs to be tightly controlled. Certain suppressors protect the plant from responding to harmless stimuli and from overreacting to pathogens. If there is a mutation in these suppressors, the immune system can actually do more damage than good,” he added.ne way that plants fight pathogens is through effector-triggered immunity (ETI), which relies on the detection of pathogen effector proteins (proteins that are deployed by pathogens to interfere with the plant immune system).

After the detection of a pathogen, specific proteins in the plant, known as resistance proteins, elicit an effective defense response.

The plants’ resistance proteins are regulated by suppressors to achieve minimal side effects to the plant while providing optimal responses to pathogens.

However, when the ETI is overly activated, it can cause stunted growth and poor seed production.n the study, MU researchers examined plants with genetic mutations that resulted in heightened plant immunity.

By examining this mutation, researchers were able to identify specific genetic components that may negatively regulate the immune system and thus contribute to an appropriate immune response.The general control of effector-triggered signaling is poorly understood,” Gassmann said.

“Better insight into the immune system response will allow us to develop plants with more durable safeguards against pathogens,” he added. (ANI)

Novel vaccine strategy may offer protection against flu viruses

Washington, May 19 (ANI): Researchers at the University of Pittsburgh suggest that vaccines made up of virus-like particles (VLPs) could provide stronger and longer-lasting protection against flu viruses than conventional vaccines.

VLP vaccines can be developed and produced twice as quickly as conventional vaccines, the researchers said.

In early clinical trials, VLP vaccines appear to provide complete protection against both the H5N1 avian influenza virus and the 1918 Spanish influenza virus, said Ted Ross, an assistant professor at the University of Pittsburgh’s Center for Vaccine Research.

Adopting the new vaccine strategy may allow public health officials to respond more quickly to emerging influenza pandemics, say researchers.

The current injectable vaccine for seasonal influenza is a trivalent, inactivated vaccine. It consists of three different influenza strains that are grown in eggs and then inactivated, or killed, by chemicals that break them into tiny pieces.

Because they no longer look like the circulating virus, conventionally made vaccines strains do not elicit as strong an immune response as VLP vaccines.

VLPs can be quickly and easily produced in several ways, including growing them in cell cultures or in plants.

Also, if the genes in the disease virus are identified, then researchers can generate particles for a vaccine without an actual sample of the agent.

“The sequence for the recent H1N1 ‘swine flu’ virus was online and available to scientists long before physical samples could be delivered,” r. Ross said.

“It would have been possible to produce VLPs in quantity in as little as 12 weeks while conventional vaccines require physical samples of the virus and production can take approximately nine months,” he added.

The study has been presented at the 109th General Meeting of the American Society for Microbiology in Philadelphia. (ANI)

UGC wants ragging treated on par with rape

CHENNAI: In an attempt to stamp out ragging in educational institutions, University Grants Commission (UGC) has decided to recommend that ragging be
treated as a cognizable offence on par with rape.
Significantly, even “freshers who do not report the incidents of ragging either as victims or as witnesses shall also be punished suitably,” says the UGC’s policy draft on curbing the menace of ragging.

A senior UGC official said the regulations, when notified, would serve as a framework on the basis of which the police could take action on complaints from educational institutions and students. “The Supreme Court had entrusted to the UGC the responsibility of preparing the draft regulations,” he said.

“The institution shall strictly observe the provisions of the Act of the Central Government and the State Governments, if any, or if enacted, considering ragging as a cognizable offence under the law on a par with rape and other atrocities against women and ill-treatment of persons belonging to the SC/ST, and prohibiting ragging in all its forms in all institutions,” says the draft regulation. Currently, not many states have enacted legislation banning ragging, which makes it difficult for law enforcers to book culprits. The regulations are expected to help provide the legal framework for the police to act quickly.

According to UGC’s proposals, ragging in all its forms will be totally banned “in the entire institution, all its premises (academic, residential, sports, canteen, etc) whether located within the campus or outside, and in all means of transportation of students whether public or private”. As per the regulations, even abetting ragging is an offence.

A copy of the UGC Draft Regulations on Curbing the Menace of Ragging in Higher Educational Institutions, 2009, has been circulated to heads of all higher educational institutions in the country to elicit their opinions.

Welcoming the regulations, Anna University (Chennai) vice chancellor P Mannar Jawahar said it will definitely deter senior students from harassing freshers. “Just as a mother has to handle a new born baby carefully learning the nuances of lifting the baby, senior students too should know how to behave with freshers. But when they exceed their limits, such stringent action will serve as a deterrent,” he said.

To facilitate easy reporting about ragging, students should have unrestricted access to mobile phones and public phones in hostels and campuses, except in classrooms, seminar halls and libraries where jammers will be installed, the draft suggests. The apex higher education body has also promised financial incentives to institutions which succeed in putting an end to the menace that has taken the lives of dozens of young students over the years.

In another significant decision, the UGC has directed all private commercially managed lodges and hostels (in and around the vicinity of educational institutions) to register with local police stations. “Local police, local administration and the institutional authorities shall ensure vigil on incidents that may come within the definition of ragging and shall be responsible for action in the event of ragging in such premises…managements of such private hostels shall be responsible for not reporting cases of ragging in their premises,” the regulation has said.

9/11 masterminds hiding in Pakistan, Afghanistan: Obama

New York, Mar 28 (ANI): President Barack Obama Pak-Afghan policy, which could be his signature foreign policy effort, said that al Qaeda terrorists are hiding in Pakistan and Afghanistan and he wants them to defeat them in their safe havens to prevent their return in future.

Obama said he would increase aid to Pakistan and would, for the first time, set benchmarks for progress in fighting al Qaeda and the Taliban in both countries, the New York Times reported.

“The United States of America did not choose to fight a war in Afghanistan. Nearly 3,000 of our people were killed on Sept. 11, 2001, for doing nothing more than going about their daily lives,” Obama said.

“So let me be clear: Al Qaeda and its allies – the terrorists who planned and supported the 9/11 attacks – are in Pakistan and Afghanistan,” he said.

“We have a clear and focused goal to disrupt, dismantle and defeat Al Qaeda in Pakistan and Afghanistan, and to prevent their return to either country in the future,” Obama added.

Part of Obama’s plan includes sending hundreds of additional diplomats and civilian experts into the region.

Admiral Mullen, the Joint Chiefs chairman, submitted a classified review to the president, and among its 13 recommendations were to increase the number of American ground forces, with significant emphasis on “enablers,” such as the new training teams.

During the 90-minute debate last Friday afternoon, Obama, flanked by his national security adviser, General James L. Jones, on his left, and Biden on his right, went around the table to elicit the final views of his national security team.

During the debate, the senior administration officials said, Biden sought to put strict parameters on the size of the additional force deployed to Afghanistan and to ensure there was a specific mission for them. Biden also cast the debate in terms of what was achievable in Obama’s first term, administration officials said.

Obama is dispatching Admiral Mullen and Holbrooke to Afghanistan, Pakistan and India next week to explain his new strategy to leaders there, the NYT reported. (ANI)

Dog owners’ aggression rubs off on their pets: Study

Washington, Feb 18 (ANI): Dogs owned by people who use confrontational or aversive methods to train their violent pets will continue to remain aggressive, unless training techniques are modified, according to a new survey.

Researchers at University of Pennsylvania conducted a year-long survey of dog owners, which also showed that using non-aversive or neutral training methods-such as additional exercise or rewards-elicited very few aggressive responses.

“Nationwide, the No. 1 reason why dog owners take their pet to a veterinary behaviorist is to manage aggressive behaviour. Our study demonstrated that many confrontational training methods, whether staring down dogs, striking them or intimidating them with physical manipulation does little to correct improper behavior and can elicit aggressive responses,” said Meghan E. Herron, lead author of the study.

Researchers from the School of Veterinary Medicine at Penn said that primary-care veterinarians needed to advise owners of the risks associated with such training methods, and provide guidance and resources for safe management of behaviour problems.

The team produced a 30-item survey for dog owners who made behavioural service appointments at Penn Vet.

The questionnaire asked dog owners about how they had previously treated aggressive behaviour, whether there was a positive, negative or neutral effect on the dogs’ behaviour and whether aggressive responses resulted from the method they used.

Owners were also asked from where did they learn the training technique they employed.

Out of the 140 surveys completed, the most frequently listed recommendation sources were “self” and “trainers”.

Many confrontational methods like “hit or kick dog for undesirable behaviour” (43 percent), “growl at dog” (41 percent), “physically force the release of an item from a dog’s mouth” (39 percent), “alpha roll” physically-rolling the dog onto its back and holding it (31 percent), “stare at or stare down” (30 percent), “dominance down”-physically forcing the dog down onto its side (29 percent) and “grab dog by jowls and shake” (26 percent) elicited an aggressive response from at least 25 percent of the dogs on which they were attempted.

Also, dogs brought to the hospital for aggressive behaviour towards familiar people were more likely to respond aggressively to some confrontational techniques than dogs brought in for other behavioural reasons.

“This study highlights the risk of dominance-based training, which has been made popular by TV, books and punishment-based training advocates. These techniques are fear-eliciting and may lead to owner-directed aggression,” said Herron.

The study was aimed at assessing the behavioural effects and safety risks of techniques used historically by owners of dogs with behaviour problems.

The study has been published in the journal Applied Animal Behaviour Science. (ANI)

Scientists uncover gene therapy to eliminate brain tumours

Washington, Jan 13 (ANI): In laboratory and animal studies, scientists have found a 2-pronged gene therapeutic approach could facilitate tumour regression and long-term survival via selective recruitment of immune cells.

Researchers at Cedars-Sinai Medical Center have identified a sequence of molecular events that could help them recruit key immune cells called dendritic cells into areas of the brain where they are not naturally found and recognize tumor cells as targets for attack.

The researchers found that a protein – HMGB1 – released from dying tumor cells activates dendritic cells and stimulates a strong and effective anti-tumor immune response.

HMGB1 does so by binding to an inflammatory receptor called toll-like receptor 2, or TLR2, found on the surface of dendritic cells.

“Toll receptors play a major role in the immune system’s recognition of bacterial and viral components, but now we have shown that they also trigger an immune response against tumors,” said Maria G. Castro, Ph.D., co-director of Cedars-Sinai’s Board of Governors Gene Therapeutics Research Institute and one of the article’s senior authors.

He added: “Activation of Toll receptors was essential for two key stages in initiating immune responses against the tumor – the migration of peripheral dendritic cells into the brain tumor and the subsequent activation of dendritic cells and stimulation of a specific anti-tumor cytotoxic T-cell mediated response.”

For the study, the researchers used a combined gene therapeutic approach, using one protein (Flt3L) to draw dendritic cells from bone marrow into the brain tumors, and a second protein (Herpes Symplex type I Thymidine Kinase, or TK), combined with the antiviral gancyclovir to kill tumor cells and elicit long-term survival.

They found a novel mechanism by which tumor cell death in response to the treatment leads to the release of an endogenous tumor protein, HMGB1, which is essential to trigger the anti-tumor immunological cascade.

For the first time, the study showed that HMGB1 released from dying brain cancer cells activates TLR2 signaling on tumor infiltrating dendritic cells, leading to the activation and expansion of tumor-antigen specific T cells.

This caused the regression of the brain tumors and increased survival time by six months in experimental brain tumor models.

“The discovery of a central role for HMGB1 and TLR2 in overcoming immune ignorance to brain tumor antigens provides a new therapeutic approach in the fight against brain tumors. Our conclusions relating to anti-glioma immune responses have also been extended to enhancing immune responses against a number of other metastatic brain cancers, such as melanoma,’ said Pedro Lowenstein, M.D., Ph.D., director of the Board of Governors Gene Therapeutics Research Institute and co-senior author.

The researchers are now planning to test this novel therapeutic approach in a human clinical trial for recurrent brain tumours in 2009.

The study will be published in the latest issue of PLoS Medicine, an open-access online journal of the Public Library of Science. (ANI)