Yemeni al-Qaeda trying to acquire toxins for bombs: US

The Yemeni branch of al-Qaeda is trying to produce the lethal poison ricin, to be packed around small explosives for attacks against the United States, fear American counter-terrorism officials.

Classified intelligence reports have revealed that al-Qaeda’s affiliate in Yemen has been making efforts to acquire large quantities of castor beans, which are required to produce ricin, a white, powdery toxin that is so deadly that just a speck can kill if it is inhaled or reaches the bloodstream.

The New York Times quoted intelligence officials, as saying that they have collected evidence that Qaeda operatives are trying to move castor beans and processing agents to a hideaw

ay in Shabwa Province, in one of Yemen’s rugged tribal areas controlled by insurgents.

The officials say the evidence points to efforts to secretly concoct batches of the poison, pack them around small explosives, and then try to explode them in contained spaces, like a shopping mall, an airport or a subway station.

President Obama and his top national security aides were first briefed on the threat last year and have received periodic updates since then, top aides said.

Senior American officials say there is no indication that a ricin attack is imminent, and some experts say the al-Qaeda affiliate is still struggling with how to deploy ricin as an effective weapon.

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Vaccine for Meningitis C ‘wears off in early teens’

London, May 8 (ANI): According to a research done by the Oxford Vaccine Group at Oxford University, three-quarters of children that are vaccinated against Meningitis C lose their protection against the disease by their early teens.

The study of 250 children aged six to 12, presented to a European conference, looked at immunity seven years after the jab was given, reports BBC.

The group tested the children, who had all been vaccinated for protection against Meningitis C, for levels of antibodies against the bacteria in their bloodstream.

It was found that just one-fourth of the children had sufficient levels of the antibodies to give them protection against the disease.

“This study is just the latest to show that the personal protection given by meningitis C vaccines in early childhood doesn”t last forever,” lead researcher Professor Andrew Pollard told the European Society for Paediatric Infectious Diseases (ESPID) meeting in Nice, France

“And several countries have now responded to these findings by introducing teenage boosters, before protection fails in the population,” he added.

“By giving each teenager a booster dose of meningococcal vaccine as they are entering adolescence, we can ensure that they are protected when they most need it,” deputy head of the Health Protection Agency”s Vaccine Evaluation Unit in Manchester, Dr Jamie Findlow said.

“If, as a result of this research, a booster programme is introduced, we would actively encourage the introduction of this,” he added. (ANI)

Jordan’s hubby injects himself with cancer-related fake-tan drug

London, Apr 30 (ANI): Katie Price’s husband Alex Reid injects himself with a fake-tan drug, which is linked to cancer.

The recently married couple will be shocked to hear that regular use of Melanotan can lead to the killer disease.

And for Jordan, 31, it could be far more terrifying, for she has suffered her own cancer scare when she had a tumour removed from a finger.

Alex, 34, injects the drug straight into his stomach, meaning it gets into his bloodstream quickly.

However, Melanotan, which is only available on the Internet for around 25 pounds, is “untested and unlicensed”.

Its main ingredient was developed to guard against skin cancer, but medics believe that long-term use can cause the disease.

“It may give you a nice tan at the moment but we don’t know what kind of long-term effects it could have,” the Daily Star quoted Florence Palmer, from the Medicines and Healthcare Products Regulatory Service, as saying.

Alex’s habit came out via Celebrity Big Brother housemate Vinnie Jones, 45, who called it “weird”. (ANI)

Abortion clinic doctor infected women with hep C

Fears of a public health threat are being hosed down by authorities in Victoria after the revelation a doctor has been suspended from practice because 12 of his female patients have contracted Hepatitis C.

The anaesthetist has the disease and police and the Medical Practitioners Board are investigating how it was transmitted to the women.

The Department of Human Services says DNA tests have linked the strain of the virus found in the women to the strain the doctor has.

The doctor has not been named, but he was working at an abortion clinic in Melbourne between June 2008 and December 2009.

Critics say the public should have been notified about the possibility of infection far sooner.

Hepatitis C takes a long time to surface. It can cause fatal liver problems and is very hard to treat.

People with the infection can pass it on if their blood gets under the skin or into the bloodstream of another person, possibly through the use of a shared syringe.

In this instance the anaesthetist passed his Hepatitis C onto 12 women, many of whom were pregnant when they were infected with the disease between June 2008 and December last year.

At the time the anaesthetist was working at the Croydon Day Surgery in outer east Melbourne.

Health authorities do not know how it happened. They do not believe anyone else has been infected but they cannot guarantee it.

Victoria’s chief health officer, Dr John Carnie, says other people who visited the surgery are being tested.

“I mean I am pretty confident that if there were any other notified cases we would have picked them up. But for completeness sake we are going to call these other people back and get them tested as well,” he said.

Accident or deliberate?

Dr Carnie says he cannot explain how 12 people could be infected by accident.

“Accidents might involve say one or two patients, but we are dealing with a cluster of 12 patients. So at this stage there is nothing in the processes and procedures at this clinic that would enable me to explain how it happened,” he said.

He says investigations began in December when three people presented with Hepatitis C who had each been treated at the clinic over a six-month period.

Officers asked for all staff involved in surgical procedures to be tested.

“All of the staff at the time tested negative except for one of their medical practitioners who happened to be overseas at that time,” Dr Carnie said.

“So on that person’s return from overseas we arranged for that person to be tested – and this was at the beginning of February – and the results were clear, the person was Hepatitis C positive.

“We then asked the lab to conduct what are called sequencing studies. What it means is comparing the structure of a virus that you get from patients and comparing that structure with the virus that was obtained from the doctor concerned; similar to I guess doing a kind of fingerprint matching if you like.

“And the laboratory has found that there is a clear link from a structural point of view between the viruses of the three patients that we initially identified and that of the doctor.”

Notification criticism

There are concerns authorities took too long to notify the public about the possibility of infection.

National president of the Maternity Coalition, Lisa Metcalfe, says it has taken a long time for authorities to act and women are vulnerable.

“Where medical practitioners have been acting inappropriately and it has taken the Health Department some time – I mean 12 cases is a lot of women to be exposed to this kind of alleged abuse,” she said.

“It has taken a long time for them to actually act on it and to have effect, take effect and to do something about reining these medical practitioners in.”

In 2008 in the United States a district health authority in Nevada issued a public warning and called on people who had used a Las Vegas clinic over a four-year period to be tested for Hepatitis C and HIV.

The warning came after an investigation found the clinic had been responsible for unsafe anaesthesia injection practices.

The Southern Nevada Health District identified six cases of Hepatitis C at the clinic, five of which stemmed from procedures on the same day involving anaesthesia.

It said a syringe that was used to administer anaesthetics to one patient may have contaminated the vial from which the anaesthetics were drawn. Intended for single use only, the vial was subsequently reused.

The district advised 40,000 patients to contact their doctors and get tested for the disease.

Nothing like this happened in Victoria. Instead authorities went to the clinic and asked for a record of patients. Officials are gradually going through the list and contacting patients one by one.

Chief health officer Dr John Carnie says they did not want to alarm members of the public by issuing a warning in the first place back in February.

“We are in the process of starting to call people back, other people who may have had procedures at this clinic,” he said.

“We didn’t want them to be alarmed by a call from the department but we wanted them to be aware of the issue that we are dealing with, and that was the reason for making this public.”

Adelaide part of golden staph vaccine trial

Adelaide’s Women’s and Children’s Hospital is after volunteers for a trial of a vaccine aimed at preventing potentially-deadly golden staph infection.

The bacteria live on the skin of about 30 per cent of the population and are usually harmless.

But if they enter the bloodstream, it can lead to pneumonia and joint infections.

Hospital patients are susceptible if they are recovering from surgical or other wounds.

Dr Helen Marshall says the hospital wants to recruit about 50 people for the trial.

“We’re hoping to enrol adults particularly for the study, 18 to 25-year-old adults and then 50 to 85 years,” she said.

“The study is being done in many centres around Australia, we’re just one of the study centres participating.”

The hospital can be contacted on 8161 6328.

Vitamin E loaded into contact lenses may treat glaucoma

Washington, Mar 25 (ANI): Contact lenses containing vitamin E can keep glaucoma medicine near the eye where it can treat the common disease- almost 100 times longer than possible with current commercial lenses, scientists claim.

In a presentation at the 239th National Meeting of the American Chemical Society (ACS), researchers described use of vitamin E to develop contact lenses that may deliver more medication for glaucoma and perhaps other diseases to the eye.

Anuj Chauhan, Ph.D., who headed the research team, explained that glaucoma is second only to cataracts as the leading cause of vision loss and blindness in the world. Eye drops that relieve the abnormal build-up of pressure inside the eye that occurs in glaucoma, are a mainstay treatment.

“The problem is within about two to five minutes of putting drops in the eye, tears carry the drug away and it doesn”t reach the targeted tissue,” said Chauhan, who is with the University of Florida in Gainesville. “Much of the medicine gets absorbed into the bloodstream, which carries it throughout the body where it could cause side effects. Only about one to five percent of drugs in eye drops actually reach the cornea of the eye.”

Chauhan and colleagues have developed a new extended-release delivery approach incorporating vitamin E into contact lenses. The invisible clusters, or aggregates, of vitamin E molecules form what Chauhan describes as “transport barriers” that slow down the elusion of the glaucoma medication from the lens into the eye. The drug released from the lens into the eye stays in the tears far longer than the 2-5 minutes with eye drops, leading to more effective therapy.

“These vitamin structures are like ”nano-bricks”,” Chauhan said. “The drug molecules can”t go through the vitamin E. They must go around it. Because the nanobricks are so much bigger than the drug molecules – we believe about a few hundred times bigger – the molecules get diverted and must travel a longer path. This increases the duration of the drug release from the lenses.”

In research with laboratory animals, the lenses containing vitamin E nanobricks administered drugs up to 100 times longer than most commercial lenses. The lenses could be designed for continuous wear for up to a month, Chauhan said. In addition to treating glaucoma, the contacts could help other eye conditions, such as cataract and dry eye. Cataract is a clouding of the lens of the eye, and dry eye involves decreased production of tears. (ANI)

Cancer genes switched off in humans

London, Mar 22 (ANI): For the first time, researchers have used short sequences of RNA that can effectively treat skin cancer in people by silencing specific genes behind tumour production.

Mark Davis from the California Institute of Technology in Pasadena and his colleagues have used the technique, called RNA interference (RNAi), to deliver particles containing such sequences to patients with the skin cancer melanoma.

When analysing biopsies of the tumours after treatment, they found that the particles had inhibited expression of a key gene, called RRM2, needed for the cancer cells to multiply.

The researchers created the particles from two polymers plus a protein that binds to receptors on the surface of cancer cells and pieces of RNA called small-interfering RNA, or siRNA, designed to stop the RRM2 gene from being translated into protein.

The siRNA works by sticking to the messenger RNA (mRNA) that carries the gene”s code to the cell”s protein-making machinery and ensuring that enzymes cut the mRNA at a specific spot.

When the components are mixed together in water, they assemble into particles about 70 nanometres in diameter.

The researchers can then administer the nanoparticles into the bloodstream of patients, where the particles circulate until they encounter ”leaky” blood vessels that supply the tumours with blood.

The particles then pass through the vessels to the tumour, where they bind to the cell and are then absorbed.

Once inside the cell, the nanoparticles fall apart, releasing the siRNA. The other parts of the nanoparticle are so small, they pass out of the body in urine.

“It sneaks in, evades the immune system, delivers the siRNA, and the disassembled components exit out,” Natrue quoted Davis as saying.

When researchers analysed tumour samples from three of the patients who volunteered samples, they found fragments of the mRNA in exactly the length and sequence they would expect from the design of their siRNA.

And in at least one patient, the levels of the protein were lower than they were in samples of the tumours taken before treatment.

They also found that patients who were given higher doses had higher levels of siRNA in their tumours.

“The more we put in, the more ends up where they are supposed to be, in tumour cells,” said Davis.

Davis says that by targeting specific genes he hopes these treatments will not have major side effects.

“My hope is to make tumours melt away while maintaining a high quality of life for the patients. We”re moving another step closer to being able to do that now,” he said.

The study has been published in Nature. (ANI)

Chemical that ‘protects’ hearts of muscular dystrophy patients discovered

Washington, Mar 16 (ANI): University of Minnesota Medical School scientists have discovered a chemical that may, over the long term, protect the hearts of Duchenne muscular dystrophy patients – a fatal and most common form of muscular dystrophy in children.

The chemical, which Medical School scientists have termed a “molecular band-aid,” seeks out tiny cuts in diseased heart muscle. When injected into the bloodstream, the molecular band-aid finds these microscopic cuts and protects them from harmful substances so the heart muscle cells can survive and function normally. In order to be effective the chemical must be repeatedly injected, much in the same way a diabetic patient requires regular injections of insulin,

In the March 15 edition of the Journal of Clinical Investigation, Joseph Metzger, Ph.D., professor and chair of the Department of Integrative Biology and Physiology, DeWayne Townsend, D.V.M., Ph.D., assistant professor in the Department of Integrative Biology and Physiology, and colleagues showed the first ever effective long-term treatment for preventing cardiac injury and progressive heart chamber remodeling in a severely affected canine model of muscular dystrophy.

In the study, dystrophic dogs were given the molecular band-aid continuously for two months. The treatment completely blocked cardiac injury and heart disease remodeling compared to the control group of dystrophic canines receiving a placebo.

“The advance in this study is demonstrating that molecular band-aid therapy is a safe and effective approach in preventing heart damage in severely affected large animals with muscular dystrophy,” Metzger said. (ANI)

Here’s why sugar in green tea is a healthy idea

Washington, Sept 10 (ANI): A new study has shown that adding ascorbic acid and sugar to green tea can help the body easily absorb helpful compounds that help fight health problems.

Mario Ferruzzi, lead researcher and associate professor of food science and nutrition at Purdue University, insists that adding ascorbic acid to green tea would increase the absorbability of catechins found in the tea.

Catechins, a class of polyphenols common in tea, cocoa and grape, are antioxidants thought to fight heart disease, stroke, cancer, diabetes and other health problems.

Ascorbic acid, sucrose or both together increase by as much as three times the amount of catechins that can be absorbed into the bloodstream.

According to Ferruzzi, Elsa Janle, a Purdue associate research professor of foods and nutrition, and Catrina Peters, the new study also demonstrates the effectiveness of a model that could reduce the number of animals needed for these types of studies.

The model charts how the digestive stability, solubility and absorption of polyphenols changes based on modifications to a beverage’s formula.

Ferruzzi said testing with the model could allow researchers to predict how a new product formula might change the product’s properties, reducing the number of animals needed for testing to only products that showed desired characteristics in the model.

The study backed up the model study that showed adding sugar and vitamin C to green tea enhanced the body’s ability to absorb polyphenols.

Ferruzzi said that adding lemon juice or other citrus juice to tea would do the trick, or consumers could look for ready-to-drink products that contain 100 percent of the recommended amount of vitamin C or ascorbic acid on the ingredient list.

“Having that vitamin C seems to do it,” Ferruzzi said. “And if you don’t want to squeeze a lemon into your cup, just have a glass of juice with your green tea.”

The study appears in journal Food Research International. (ANI)

Fat-rich junk food may alter genes linked with type II diabetes

London, September 8 (ANI): A team of scientists in Sweden have warned that gorging too much on fat-rich junk food may cause drastic changes to a gene that helps muscle cells burn fat.

Juleen Zierath, of the Karolinska Institute in Stockholm, says that her team’s findings may help improve the scientific understanding of how type II diabetes develops in adulthood.

“Somehow, the environment plays on the genes we have,” says the lead researcher, adding that her study provides new clues to how this happens.

She says that it may be possible that the altered cells become so engorged with unburnt fat that they become “diabetic”, and stop accepting signals from the hormone insulin, which normally triggers the absorption of glucose from the bloodstream.

However, proving that components in the diet can permanently alter genes is itself a breakthrough, as it provides the first evidence that the food people eat may change the function of their DNA, a process scientifically known as “epigenetics”.

During the study, the researchers observed that the DNA itself remained unchanged, except for a masking process called methylation that can permanently mothball a gene by capping individual chemical units or bases.

Before the researchers undertook this research, they had already found in a previous study that muscle cells from people with type II diabetes showed such telltale epigenetic alterations to their DNA, particularly in the PGC-1 gene, which orchestrates metabolic programmes critical to the burning of fat in mitochondria, the chambers in cells that generate energy.

In the current study, the researchers achieved the most significant result when they exposed the healthy muscle cells to the edible fatty acid, palmitic acid.

The team found that the PGC-1 gene became methylated, just as it is in people with diabetes.

“The palmitic acid essentially switches off the gene,” New Scientist magazine quoted Zierath as saying.

She says that the fact that fat produces such an effect is highly significant, as it means that over-consumption of junk food may cause the same response.

“It suggests that if you eat a fat-rich diet, something in that – either the fat itself or the build up of metabolites – triggers the methylation of genes. The net effect is that it switches off the gene,” says Zierath.

The team’s analyses also reveal that the shutdown of PGC-1 led to inactivation of other genes vital for burning or transporting fat.

Zierath says that her team’s next step will be to find out how different diets affect the methylation status of PGC-1 and other genes vital for burning energy, hoping that their efforts will lead to the discovery of a potential mechanism by which type II diabetes develops.

A research article on her study has been published in the journal Cell Metabolism. (ANI)

Scientists find novel way to deliver cancer-fighting molecules

London, Aug 28 (ANI): Scientists from University of Iowa have found a novel way to inject cancer-fighting molecules in the bloodstream and inhibit tumour growth.

Small interfering RNA (siRNA), a type of genetic material, are known to block potentially harmful activity in cells, such as tumour cell growth.

But delivering siRNA successfully to specific cells without adversely affecting other cells has been challenging.

In the new study, the researchers have modified siRNA so that it can be injected into the bloodstream and impact targeted cells while producing fewer side effects.

The findings, which were based on animal models of prostate cancer, also could make it easier to create large amounts of targeted therapeutic siRNAs for treating cancer and other diseases.

“Our goal was to make siRNA deliverable through the bloodstream and make it more specific to the genes that are over expressed in cancer,” Nature quoted Dr Paloma Giangrande, assistant professor of internal medicine and a member of Holden Comprehensive Cancer Centre as saying.

Previous studies have shown that a compound called an aptamer can be combined with siRNA to target certain genes.

However, in the new study, the researchers trimmed the size of a prostate cancer-specific aptamer and modified the siRNA to increase its activity.

After injecting it into the bloodstream, the combination triggered tumour regression without affecting normal tissues.

Giangrande said making the aptamer-siRNA combination smaller akes it easier to produce large amounts of it synthetically,

The study results appeared in journal Nature Biotechnology. (ANI)

Fat in liver, not belly, could determine heart disease risk

Washington, Aug 25 (ANI): Measuring liver fat may be a better way to determine a person’s risk for developing diabetes and heart disease than measuring belly fat, according to researchers at Washington University School of Medicine in St. Louis.

Having too much liver fat is known as nonalcoholic fatty liver disease.

The researchers say that when fat collects in the liver, people experience serious metabolic problems such as insulin resistance, which affects the body’s ability to metabolize sugar.

They also have increases in production of fat particles in the liver that are secreted into the bloodstream and increase the level of triglycerides.

For years, scientists have noted that where individuals carried body fat influences their metabolic and cardiovascular risk.

Increased fat inside the belly, known as visceral fat, is linked to an higher risk of diabetes and heart disease.

“Data from a large number of studies shows that visceral fat is associated with metabolic risk, which has led to the belief that visceral fat might even cause metabolic dysfunction,” says study’s lead author Samuel Klein.

“However, visceral fat tracks closely with liver fat. We have found that excess fat in the liver, not visceral fat, is a key marker of metabolic dysfunction. Visceral fat might simply be an innocent bystander that is associated with liver fat,” he added.

Klein says most of our body fat, called subcutaneous fat, is located under our skin, but about 10 percent is present inside the belly, while much smaller amounts are found inside organs such as the liver and muscle.

In the study, the researchers compared obese people with elevated and normal amounts of liver fat. All subjects were matched by age, sex, body mass index; percent body fat and degree of obesity.

Through careful evaluations of obese people with different amounts of visceral fat or liver fat, Klein’s team determined that excess fat inside the liver identifies those individuals who are at risk for metabolic problems.

The study has been published online in the journal PNAS Early Edition. (ANI)

Scientists identify how meningitis bacteria invade the brain

Washington, Aug 19 (ANI): Scientists in the U.S. have discovered that a specific protein on the surface of a common bacterial pathogen allows the bacteria to leave the bloodstream and enter the brain, initiating the deadly infection known as meningitis.

The new finding may lead to the development of improved vaccines to protect those most vulnerable, including young infants and the elderly.

“Streptococcus pneumoniae, commonly known as pneumococcus, is responsible for half the cases of bacterial meningitis in humans,” said the study’s senior author, Victor Nizet, MD, professor of paediatrics and pharmacy at the University of California, San Diego’s School of Medicine and Skaggs School of Pharmacy and Pharmaceutical Sciences.

Meningitis develops when bacteria penetrate the “blood-brain barrier.”

The blood-brain barrier, comprised of a single layer of highly specialized microvascular endothelial cells, prevents most large molecules from entering into the cerebrospinal fluid, preserving an optimal biochemical environment for brain function.

The research team examined the functions of a protein known as NanA in order to discover how an entire bacterium can breech the blood-brain barrier and gain access to the central nervous system.

NanA is produced by all strains of pneumococcus and displayed prominently on the bacteria’s outer surface.

Through genetic manipulations, the researchers were able to remove the entire NanA protein, or just specific sections of the molecule, from the pathogen.

They found that while normal pneumococci were able to bind, enter and penetrate through human brain microvascular endothelial cells, mutant bacteria lacking the NanA protein -or those expressing only a truncated version of the protein – largely lost these abilities.

Conversely, when the full-length pneumococcal NanA protein was cloned and expressed on the surface of a nonpathogenic laboratory strain, the transformed bacteria gained the ability to bind and enter the same endothelial cells.

Satoshi Uchiyama, MD, a postdoctoral fellow in the Nizet Laboratory and lead author on the study, said: “Our tissue culture studies showed that the NanA protein was both necessary and sufficient for bacterial penetration of the blood brain barrier endothelial cells.”

“After infecting mice intravenously, we also found that far fewer NanA-deficient bacteria left the bloodstream and entered the brain, in comparison to mice infected with the normal pneumococcus,” Uchiyama added.

NanA is best known as an enzyme that cleaves and releases the sugar molecule known as sialic acid, which is present in abundance on the surface of all human cells.

While this enzymatic activity played a small part in promoting NanA-mediated blood-brain barrier interactions, a much stronger role was identified for the outer tip of the protein.

This tip seems to directly attach to the brain microvascular endothelial cells and then stimulate them to take in the pneumococcus.

According to Nizet, because NanA is expressed on the surface of all pneumococcal strains, it is an attractive candidate to include in a universal protein-based vaccine against pneumococcal infection.

The study is available online in the Journal of Experimental Medicine. (ANI)

Why retroviruses like HIV get easily acquainted with uninfected neighbours

Washington, July 28 (ANI): Yale University researchers have found out why retroviruses like HIV can get easily transmitted when they are next to uninfected cells than if they are floating free in the bloodstream.

The researchers, led by Dr. Walther Mothes at Yale, have made movies of viral activity within cells that help explain why cell-to-cell transmission is so efficient, and provide potential targets for a new generation of AIDS drugs.

“Cell-to-cell transmission is a thousand times more efficient, which is why diseases such as AIDS are so successful and so deadly. And because the retroviruses are already in cells, they are out of reach of the immune system,” said Mothes.

By using imaging technology that can track individual particles of virus in real time, the researchers discovered that infected cells could specifically produce viruses at the point of contact between cells.

They also observed that ten times more of these particles are found at these cellular poles than elsewhere at the surface of cells.

Scientists claimed that the ability of infected cells to specifically produce viruses only at cell-cell interfaces explains how viruses spread so efficiently.

The researchers also identified a possible weakness in the transmission chain.

The team found that viruses express a sticky protein that docks with uninfected cells and then attracts viral assembly to these sites.

If this adhesion molecule lacked a “cytoplasmic tail,” then it would mean that the viral particles did not assemble at the jumping off point between cells.

Mothes is expecting that many more such targets will be identified as scientists work out the mechanics of cell-to-cell transmission.

“We are just opening the door to this whole process. It is a black box, and many, many cellular factors have to be involved in making this happen. Our hope is that somewhere down the road we will have a completely new anti-viral strategy based on targeting cell-to-cell transmission,” said Mothes.

The study has been reported in the open access journal PLoS Biology. (ANI)

Inflammation may lead to Alzheimer’s disease

Washington, July 9 (ANI): A new study has revealed that inflammation might lead to development of Alzheimer’s disease.

Two research studies published by William A. Banks has shown how inflammation triggers disease, and how can it be treated.

It is believed that the toxic levels of amyloid beta protein, the substance responsible for Alzheimer’s disease, accumulate in the brain because a pump that pushes it into the blood and past the blood-brain barrier malfunctions.

The blood-brain barrier is a system of cells that regulates the exchange of substances between the brain and the blood.

The blood-brain barrier transporter known as LRP is the pump that removes amyloid beta protein from the brain and into the bloodstream.”LRP malfunctions like a stop light stuck on red, and keeps amyloid beta protein trapped in the brain,” said Banks.

Inflammation, which is part of the body’s natural immune response, occurs when the body activates white blood cells, and produces chemicals to fight infection and invading foreign substances.

“We induced inflammation in mice and found that it turned off the LRP pump that lets amyloid beta protein exit the brain into the bloodstream,” Banks said.

“It also revved up an entrance pump that transports amyloid beta into the brain. Both of these actions would increase the amount of amyloid beta protein in the brain,” he added.

Banks said that treatment with drug indomethacin prevented inflammation from turning off the LRP (exit pump). (ANI)

How obesity leads to diabetes

Washington, July 9 (ANI): Monash University researchers have found how obesity leads to type 2 diabetes – a finding that could lead to the design of a drug to prevent the disease.

Though obesity is associated as a leading cause of diabetes, no one has understood the exact mechanism of how obesity inhibits the body’s ability to use insulin leading to type 2 diabetes until now.

Now, the research team, led by Associate Professor Matthew Watt, discovered that fat cells release a novel protein called PEDF (pigment epithelium-derived factor), which triggers a chain of events and interactions that lead to development of Type 2 diabetes.

“When PEDF is released into the bloodstream, it causes the muscle and liver to become desensitised to insulin. The pancreas then produces more insulin to counteract these negative effects, ” Watt said.

“This insulin release causes the pancreas to become overworked, eventually slowing or stopping insulin release from the pancreas, leading to Type 2 diabetes.

“It appears that the more fat tissue a person has the less sensitive they become to insulin. Therefore a greater amount of insulin is required to maintain the body’s regulation of blood-glucose.

“Our research was able to show that increasing PEDF not only causes Type 2 diabetes like complications but that blocking PEDF reverses these effects. The body again returned to being insulin-sensitive and therefore did not need excess insulin to remain regulated,” Watt added.

The findings were published today in respected journal Cell Metabolism. (ANI)

20 cholesterol-regulating genes identified

Washington, July 8 (ANI): Scientists at the European Molecular Biology Laboratory (EMBL) and the University of Heidelberg, Germany, have identified 20 genes that play a vital role in maintaining cholesterol balance.

The researchers believe that the newly identified genes may help uncover the mechanisms that regulate cholesterol levels, and lead to new treatments for cholesterol-related diseases.

“This finding may open new avenues for designing targeted therapies, for example by looking for small molecules that could impact these genes,” said Heiko Runz, whose group at the University Clinic Heidelberg carried out the research together with Rainer Pepperkok’s lab at EMBL.

High levels of cholesterol in the bloodstream are a major risk factor for atherosclerosis and coronary heart disease.

During the study, the researchers deprived isolated human cells of cholesterol, and then looked at the whole genome to find the genes that react to changes in cholesterol levels by altering their expression.

With a microscope, they then observed what effect switching off different genes had both on cholesterol uptake and on the total amount of cholesterol inside cells.

Of the 20 genes the scientists identified as involved in regulating cholesterol levels and uptake, 12 were previously unknown.

The scientists are now trying to discover exactly how the novel genes regulate cholesterol levels inside cells, as well as looking at patients to determine whether these genes (or alterations in them) do constitute risk factors, and investigating if and how they could be useful drug targets.

The study appears in journal Cell Metabolism. (ANI)

Jacko died in his sleep, claims his personal doc

London, June 30(ANI): Michael Jackson’s personal doctor has denied giving him drugs in the hours before his death, saying the icon died in his sleep.

Dr Conrad Murray’s lawyer has suggested that Jackson could have injected himself with painkillers or taken pills that made him stop breathing, The Daily Express reports.

Reports have come that the legend had potent painkiller Demerol – also known as Pethidine – in his bloodstream when he died.

Dr Murray’s lawyer Ed Chernoff defended his client saying: “He has never ever prescribed or administered Demerol to Michael Jackson… Whether Michael Jackson injected drugs is not something that we can discuss until the toxicology report comes back.”

Dr Murray has also been questioned by Los Angeles Police as to why he gave emergency heart massage on a bed instead of a firm surface, as recommended by experts.

His spokeswoman explained: “As Jackson is frail and small and heart pumping can be quite forceful, Murray thought the bed was better.” (ANI)

Molecule-size capsules can deliver drugs to targeted cells 100pct efficiently

Washington, June 26 (ANI): Scientists have devised a way to make tiny containers about the size of a virus that can deliver medicines to targeted cells in the bloodstream with almost 100 percent efficiency.

The breakthrough achieved by a collaborative team of Cornell and Shenzhen University researchers gives new hope that this technique may one day be used to deliver vaccines, drugs or genetic material to treat cancer and blood and immunological disorders.

“We can introduce just about any drug or genetic material that can be encapsulated, and it is delivered to any circulating cells that are specifically targeted,” said Michael King, Cornell associate professor of biomedical engineering, who co-authored the study with lead author Zhong Huang, a former Cornell research associate who is now an assistant professor at the Shenzhen University School of Medicine in China.

The technique involves filling the tiny lipid containers, or nanoscale capsules, with a molecular cargo and coating the capsules with adhesive proteins called selectins that specifically bind to target cells.

A shunt coated with the capsules is then inserted between a vein and an artery. Much as burrs attach to clothing in a field, the selectin-coated capsules adhere to targeted cells in the bloodstream.

After rolling along the shunt wall, the cells break free from the wall with the capsules still attached and ingest their contents.

The study shows that since only the targeted cells ingest the contents of the nanocapsules, the technique could greatly reduce the adverse side effects caused by some drugs.

The study also shows that genetic material can be delivered to targeted cells to turn off specific genes and interfere with processes that lead to disease.

The researchers filled nanocapsules with a small-interfering RNA (siRNA) and targeted them to specific circulating cells. When the targeted cells ingested the capsules, the siRNA turned off a gene that produces an enzyme that contributes to the degradation of cartilage in arthritis.

King said that in a similar manner, the method could be used to target the delivery of chemotherapy drugs, vaccine antigens to white blood cells, specific molecules that mitigate autoimmune disorders and more.

The research has been published online at the Web site of the journal Gene Therapy. (ANI)

Scientists identify new glucose-regulating protein linked with diabetes

Washington, May 29 (ANI): Scientists have found that a specialized protein in human muscles is linked with the process that clears glucose out of the bloodstream.

The finding by researchers at the University of California, San Francisco, and collaborators at Harvard Medical School, could shed light on what goes wrong in type 2 diabetes on a cellular level.

If the function of this protein (apparently absent in mice) is established, it could pave the way for future study and possible therapies for diabetes.

“Much has been learned from mouse models about glucose metabolism that is relevant to human diabetes, but what happens on a cellular level is now found to be different between the two species. This research shows one significant species-specific difference that will influence our understanding of mechanisms of type 2 diabetes,” said Dr. Frances Brodsky, senior author on the paper.

The researchers said that in humans, muscles play a key role in clearing glucose from the bloodstream-a process normally controlled by insulin, which stimulates the muscle cells to import glucose by means of a system known as the GLUT4 glucose transporter.

Usually GLUT4 is stored inside both human and mouse muscles in a special compartment that releases it upon insulin stimulation. Fat cells also form a GLUT4 compartment and take up glucose in response to insulin.

But, in type 2 diabetes, the muscle and fat cells fail to respond appropriately to the insulin and the GLUT4 compartment is abnormal-a function thought to be identical across mammal species.

The current research identified a protein in both human muscle and fat cells-called CHC22-that appears to control the formation of the GLUT4 storage compartments.

The team determined that this protein is a specialized form of an omnipresent housekeeping protein called clathrin, which is known to be instrumental in moving proteins between cellular compartments.

The researchers observed that CHC22 was associated with the abnormal GLUT4 compartments in muscles from diabetic patients, which, for an unknown reason, do not mobilize to the muscle cell surface when stimulated by insulin.

She particularly said that while mice have an insulin-responsive GLUT4 compartment, they lack the CHC22 protein.

Thus, this work has implications for developing better models for the study of type 2 diabetes.

The study has been published in the journal ‘Science’. (ANI)